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Research Scientist | Research Associate Resume

Desired Industry: Biotech SpiderID: 83259
Desired Job Location: Williamsville, New York Date Posted: 8/6/2021
Type of Position: Full-Time Temporary Availability Date: JULY 2021
Desired Wage: 50,000
U.S. Work Authorization: Yes
Job Level: Management (Manager, Director) Willing to Travel: Yes, 25-50%
Highest Degree Attained: Bachelors Willing to Relocate: Yes

Success-driven and highly analytical professional, offering broad expertise in all facets of scientific research and project development operations and data analysis; backed by broad-based work experience in cell biology, molecular biology, and immunology research; quantitative and cell-based assay development; as well as biobanking.

»»»Research Experience

»»»Cellecta Inc., Mountain View, CA
»»Research Scientist Apr 2015–Jul 2021

•Executed lentiviral packaging (HIV) and transduction of cDNA, clustered regularly interspaced short palindromic repeats (CRISPR/Cas9), and short-hairpin RNAs (shRNA) constructs for contract research organization (CRO) services
•Created quantitative assays to assess gene editing efficiency in target cells, along with cell-based assays to evaluate expression and biological functions of specific genes
•Played a pivotal role in the implementation of RNA- sequencing workflow platform, including RNA extraction, quantitative real-time polymerase chain reaction (qRT-PCR), and next-generation sequencing (NGS)
•Drafted technical reports regarding assay development and optimization as well as the standard operating procedures (SOPs) for assay performance
•Ensured maintenance of accurate and detailed inventory, propagation, and cryopreservation of mammalian cell lines and engineered cells for cell bank
•Facilitated training of new employees on aseptic techniques and methodologies for mammalian cell culture

»»»Everon Biosciences, Buffalo, NY
»»Research Scientist Jul 2012–Mar 2015

•Conducted research focused on pharmacologically targeting senescence cells as a therapeutic modality for age-related diseases
•Implemented screening of small molecules in senescent cells for proper identification of anti-aging drug targets
•Determined optimum cellular screening strategies through collaboration with discovery program teams and research leadership
•Led the engineering, maintenance, and preparation of transduced cells for ex vivo and in vivo studies
•Contributed to the discovery of several lead compounds that showed a rejuvenating effect in aging mice

»»»Benaroya Research Institute, Seattle, WA
»»Research Technician III Jan 2005-Apr 2009

•Identified antigen specific cells associated with pathogens, tumors, allergens, and autoimmune diseases through tetramer guided epitope mapping (TGEM)
•Purified recombinant MHC Class II proteins from Drosophila S2 insect cells using affinity chromatography
•Performed isolation and immunophenotyping of PBMCs and primary CD4+ T cells from patient blood samples using density gradient centrifugation and MACS
•Supervised the development, execution, and interpretation of analytical assays with tetramers, including cytokine secretion, CFSE proliferation, cytotoxicity assays, and multiparametric flow cytometry

»»»University of California San Diego, San Diego, CA
»»Staff Research Associate | Laboratory Manager Mar 2001–Dec 2004

•Conducted gene cloning, nucleic acid purification and expression that involved bacterial culture, transformation, plasmid DNA isolation, and electroporation
•Utilized B-gal, GFP, Luciferase, and SEAP in performing transcription factor reporter and binding assays
•Generated transgenic constructs for mice injections and genotyping
•Efficiently handled general laboratory safety and maintenance, such as ordering and stocking supplies, maintaining equipment, and ensuring compliance with federal and state regulations


•Bachelor of Arts in Biology, June 1995
University of California, Santa Cruz- Santa Cruz, CA

»»»Areas of Expertise

•Lentiviral Packaging and Transduction
•Mammalian Cell Culture
•CRISPR-based Genome Editing
•Immune Cell Function Assays
•Molecular Biology Techniques Application
•DNA, RNA, and Protein Extraction
•Vector Design and Cloning
•Fluorescent-Activated Cell Sorting (FACS)
»»»Technical Skills

•Operating Systems
•Macintosh | Windows
•Systems and Software
•FileMaker Pro Advanced | Google Suite | Microsoft Office Suite (Word, Excel, PowerPoint)
Research Tools
•FlowJo Software | Geneious| GraphPad Prism | SnapGene | Quartzy

Additional Information:
»»»Selected Publications

»»Peer reviewed journal articles

Kwok, W. W., Roti, M., DeLong, J. H., Tan, V., Wambre, E., James, E. A., and Robinson, D. (2010). Direct ex vivo analysis of allergen-specific CD4+ T cells. The Journal of Allergy and Clinical Immunology, 125(6), 1407–1409. PMID: 20513526.

Kwok, W. W., Yang, J., James, E., Bui, J., Huston, L., Wiesen, A. R., and Roti, M. (2008). The anthrax vaccine adsorbed vaccine generates protective antigen (PA)-Specific CD4+ T cells with a phenotype distinct from that of naive PA T cells. Infection and Immunity, 76(10), 4538-4545. PMID: 18678674.

Roti, M., Yang, J., Berger, D., Huston, L., James, E. A., & Kwok, W. W. (2008). Healthy human subjects have CD4+ T cells directed against H5N1 influenza virus. The Journal of Immunology, 180(3), 1758-1768. PMID: 18209073.

Yang, J., Danke, N., Roti, M., Huston, L., Greenbaum, C., Pihoker, C., James, E., and Kwok, W. W. (2008). CD4+ T cells from type 1 diabetic and healthy subjects exhibit different thresholds of activation to a naturally processed proinsulin epitope. Journal of Autoimmunity, 31(1), 30-41. PMID: 18385016.

Yang, J., James, E., Roti, M., Huston, L., Gebe, J. A., and Kwok, W. W. (2008). Searching immunodominant epitopes prior to epidemic: HLA class II-restricted SARS-CoV spike protein epitopes in unexposed individuals. International Immunology, 21(1), 63-71. PMID: 19050106.

James, E. A., Bui, J., Berger, D., Huston, L., Roti, M., and Kwok, W. W. (2007). Tetramer-guided epitope mapping reveals broad, individualized repertoires of tetanus toxin-specific CD4+ T cells and suggests HLA-based differences in epitope recognition. International immunology, 19(11), 1291-1301. PMID: 17906339.

Lu, Q., Liu, X., Trama, J., Roti, M., Go, W. Y., & Ho, S. N. (2004). Identification of the cytoskeletal regulatory protein α-adducin as a target of T cell receptor signaling. Molecular Immunology, 41(4), 435-447. PMID: 15163540.

Go, W. Y., Liu, X., Roti, M. A., Liu, F., and Ho, S. N. (2004). NFAT5/TonEBP mutant mice define osmotic stress as a critical feature of the lymphoid microenvironment. Proceedings of the National Academy of Sciences, 101(29), 10673-10678. PMID: 15247420.

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