Research Associate Resume

Desired Industry: Biotech	SpiderID: 28602
Desired Job Location: Sunnyvale, California	Date Posted: 9/9/2009
Type of Position: Full-Time Permanent	Availability Date: 09/09/2009
Desired Wage:
	U.S. Work Authorization: Yes
Job Level: New Grad/Entry Level	Willing to Travel: No
Highest Degree Attained: Bachelors	Willing to Relocate: No

Objective:
Seeking a biological research or laboratory position in the biomedical and/or biotech industries

Experience:
Contract Scientist
2007 - 2009
[See breakdown of each position below.]
Over the last two years, I have worked in a steady series of contract roles. This diverse experience has allowed me to rapidly expand my technical & problem-solving skills, perform well within different team cultures, and effectively multi-task among shifting priorities. Upon request, I can provide several references who will attest to the quality of my work during this period.

Affymetrix, Fremont, CA (2nd Contract Position with Affymetrix)
Feb – Jun 2009
Research Associate, Research and Development
Responsible for performing genetic and immunological experiments and analyzing results on customer samples. Responsible for supporting new product development. Also responsible for the establishment of stable cell lines.
• Conducted Procarta Cytokine, DC and Transcription Factor Plex assays to evaluate biological samples
• Performed Quantikine Porcine, TNF-alpha and Transcription Factor ELISAs to analyze samples
• Purified antibodies by using desalt columns, Protein G Plus and the Melon Gel Purification Kit
• Biotinylated mouse antibodies and calculated molar ratios
• Conjugated microspheres to human and mouse antibodies for analysis
• Prepared buffers, bead mixtures, diluents, detection antibody mixtures and SAPE
• Transfected cell lines with a luciferase reporter plasmid and isolated stable transfectant clones
• Assessed reporter gene induction by performing luciferase assays and monitoring reporter stabilities
• Tested and developed new Procarta products. Wrote protocols and updated SOPs
• Recorded detailed laboratory observations, procedures and results in a laboratory notebook
• Created detailed laboratory protocols using the Bioplex software
• Responsible for calibrating and repairing the Luminex machine

Baxter, Hayward, CA (Extended Contract)
Mar 2008 – Jan 2009
Laboratory Associate, Quality Control Immunology
Responsible for conducting biological, chemical and physical analyses on pharmaceutical samples.
• Performed ELISA, SDS-PAGE, Bradford assay, protein concentration absorption, chromogenic functionality assay and other experiments on samples
• Analyzed assay results of biologics, drugs and pharmaceutical samples
• Prepared buffers, staining solutions, diluents and cleaning solutions for each experiment
• Analyzed and interpreted data using the Biotek ELX808 plate reader, the Beckman DU-800 spectrophotometer, and the molecular dynamics densitometer SI
• Calculated the average, standard deviation, coefficient of variation and percent recovery of samples
• Completed test forms & detailed lab reports. Documented test procedures & interpreted test results
• Ensured compliance with FDA, GLP, QSR and cGMP regulations
• Maintained a minimal error rate for each experiment as required by SOPs
• Performed laboratory and manufacturing audits and updated SOPs
• Performed equipment maintenance, calibration and repair

Affymetrix, South San Francisco, CA (1st Contract Position with Affymetrix)
Feb - Mar 2008
Research Assistant
Responsible for washing, staining and scanning GeneChip Mapping 500K probe arrays. Also responsible for bleaching the fluidics stations and running the 500K Fluidics machines.
• Prepared wash and stock buffers. Measured the pH and conductivity of each buffer
• Prepared and analyzed the anti-streptavidin solution and array holding buffer
• Primed the fluidics station by programming the 500K Fluidics machines
• Made the stain buffer, SAPE and antibody solution mixes for staining arrays
• Washed and stained the 500K probe arrays using the 500K Fluidics machines
• Processed the solutions by pipetting and centrifuging the array samples
• Scanned the 500K probe arrays using the GeneChip Scanner 3000 7G laser
• Performed fluidics station bleaching by using the GeneChip Operating Software

Stanford Genome Technology Center, Stanford School of Medicine, Palo Alto, CA (Contract Position)
Jan - Feb 2008
Research Assistant
Responsible for conducting emulsion PCR and 454 pyrosequencing experiments. Also responsible for maintaining an inventory of reagents and recording detailed laboratory procedures.
• Performed emulsion PCR and 454 pyrosequencing experiments using yeast and algae DNA
• Amplified single strands of DNA to millions of strands by programming PCR thermocycler machines
• Segregated each DNA capture bead to a microreactor to amplify DNA by emulsion PCR
• Selected amplified DNA capture beads by using magnetic particle collectors
• Programmed thermocycler machines to anneal sequencing primers to amplified DNA
• Prepared the packing and enzyme beads and loaded the reagents into the sequencing cassette
• Launched the sequencing runs by programming the Genome Sequencer FLX Instrument
• Maintained an inventory of the emulsion PCR and 454 pyrosequencing reagents
• Documented and updated detailed laboratory protocols for emulsion PCR and 454 pyrosequencing

Intuitive Surgical, Sunnyvale, CA (Contract Position)
Dec 2007 - Jan 2008
Product Quality Assurance Analyst
Responsible for testing, cleaning and analyzing monopolar electrocautery shears for precision, durability and dexterity.
• Operated the da Vinci surgical system. Tested shears for precision by cutting meat samples
• Performed tests that involved snapping, blunt dissections and cautery to test for durability
• Analyzed the tips, covers and cables of the instruments to test for tensile strength
• Autoclaved and sterilized the instruments in enzymatic solution using the ultrasonic cleaning machine

Stanford Research Institute, Menlo Park, CA (Contract Position)
Oct - Nov 2007
Laboratory Technician, Environmental Health and Safety
Responsible for scanning and documenting chemicals at SRI’s R & D divisions.
• Scanned and organized chemicals by room and building
• Entered chemical barcodes into SRI’s computer database
• Documented chemical names, properties and manufacturing information

Education:
Bachelor of Science, Molecular Biology, Sept 2007
University of California, San Diego, CA, GPA 3.0

Skills:
Molecular Biology
o electrophoresis, restriction enzyme digest, nucleic acid & recombinant DNA plasmid purification
o emulsion PCR, 454 pyrosequencing, Transcription Factor ELISA, Transcription Factor Plex

Biochemistry
o protein & antibody purification, A280, DC & Bradford, Northern/Southern/Western Blotting
o Procarta Cytokine assay, TNF-alpha & Quantikine Porcine ELISAs, conjugation, biotinylation

Cell Culture
transfection, induction, trypsinization, making frozen stock, luciferase assay

Other
o operating robotic surgical systems, sewing heart tissue, isolating veins and arteries
o chromogenic assay, HPLC chromatography, NMR & IR spectroscopy, C/C++ & Java programming

Additional Information:
Mathematics Award, Language Arts & History Award, Art Design Award, Drawing & Painting Award

Reference:
Available upon request.

Candidate Contact Information:

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