research associate Resume

Desired Industry: Biotech	SpiderID: 2150
Desired Job Location: Fremont, California	Date Posted: 3/22/2005
Type of Position: Full-Time Permanent	Availability Date:
Desired Wage:
	U.S. Work Authorization: Yes
Job Level: Experienced with over 2 years experience	Willing to Travel: Yes, Less Than 25%
Highest Degree Attained: Doctoral	Willing to Relocate: Yes

Experience:
1.University of Southern California and University of California, Irvine
Postdoctoral fellow
September 2002- March 2004

Worked on elucidating the molecular basis of neuron apoptosis, development
of novel molecular pharmacological interventions for neuro-degenerative disease,
and generating transgenic mice.

Laboratory skills:
*Transgene cloning and purification for transgenic mice by gradient ultracentrifugation
*Endonuclease activity assay
*Knock-out and transgenic mice genotyping
*Fetal rat brain dissection and primary neuron culture
*RT-PCR
*Primary neuron transfection
*Immunoprecipitation
*In vitro transcription

II. Loma Linda University
Postdoctoral fellow
February 2001-June 2002

Worked on phage display trying to establish
an efficient targeted gene delivery system for
prevention and treatment of type I diabetes.

Laboratory skills:
* Plaque Assay of T7 Bacteriophage
* Gene Cloning and Expression Using T7 and M13 Phage-based Vector
* In-vitro and in-vivo Phage Library Biopanning
* Expression and Detection of Luciferases

III. Study on human nucleotide excision repair (hNER),
University of Texas Medical Branch, Galveston, TX 77555.
September 1999 -- February 2001. Postdoctoral fellow
in the laboratory of Assistant Professor Yue Zou.

Worked on establishment of a cell-free hNER system
and construction of a variety of damaged DNA substrates;
also on cloning and over-expression of several hNER enzymes,
in eukaryotic and/or prokaryotic system(s).

Laboratory skills:
* Homogenization of Cultured Cells and Extraction of mRNA
* PAGE Followed by Western Blot and Detection of Protein Samples
Derived from Lysates of Cultured cells
* Gene Cloning and expression in a baculovirus system
* Mammalian and Insect Cells Culture
* Stable Transfection of Insect Cells to Over-express Cloned Genes
* Protein Purification from Over-expressing Systems
* Construction of Damaged DNA Substrates
* DNA Sequencing

Education:
Doctor of Philosophy
Shanghai Medical University
Shanghai, 200032, China
9/1994 - 9/1997

Master of Science
Shandong Medical University
Ji'nan, 250011, China
9/1991 - 7/1994

Doctor of Medicine
Qingdao Medical College
Qingdao, 260003, China
9/1986 - 7/1991

Additional Information:
Computer Skills & Languages:

MS Windows, MS Office, HTML

Fluent spoken/written English, spoken German and French

Candidate Contact Information:

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