research associate Resume

Desired Industry: Biotech	SpiderID: 20384
Desired Job Location: San Jose, California	Date Posted: 3/25/2008
Type of Position: Full-Time Permanent	Availability Date: immediate
Desired Wage:
	U.S. Work Authorization: Yes
Job Level: Experienced with over 2 years experience	Willing to Travel: Yes, Less Than 25%
Highest Degree Attained: Bachelors	Willing to Relocate: No

Objective:
RESEARCH ASSOCIATE
Biochemistry major with over 7 years professional experience in cell biology, molecular biology, study design, measurement and data collection. Strong ability to address and resolve products’ problems with proven data along with excellent writing, analytic, and oral presentation skills. High level of initiative and works well both independently and as part of a team. Broad technical knowledge with hands-on experience in manufacturing various products from start to finish according to SOP in ISO-certified environment.

Experience:
CLONTECH LABORATORIES, Mountain View, CA. 06/2000 to 01/2008

Laboratory duties:
• Purified Antibodies for glass array printing
• Performed in process testing of control cDNA PCR template and quality control testing of PCR and library construction kits.
• Performed protein extraction from induced Hela cells for TransFactor kit manufacturing, which can be used in studying transcriptional regulation in different cell lines and tissues.
• Manufactured electrocompetent and chemical competent cells
• Prepared chromaspin matrice, reagents, formulations and custom orders including yeast media, reagents, buffers, solutions, and gel plates for Production and R&D
Other duties:
• Assisted R&D scientists and QC in trouble-shooting projects. Assisted with total and poly A+ RNA dilution project. Assisted to manufacture control cDNA template, DNA polymerase mix.
• Performed receiving and inspection activities for manufacturing materials, and maintained inventory control
• Wrote and revised protocols (MBRs, SOPs, WIs, etc) in compliance with ISO 9001 certification
• Generated reports to monitor productivity and customer satisfaction
• Validated and maintained equipments used including autoclaves, pH meters, conductivity meter, analytical and top-loading balances, pipetmen, motorized stirrer, and stir plates

AVOCET MEDICAL, INC, San Jose, CA. 01/1999 to 06/2000
Manufacturing Chemist

• Laboratory Duties: made thromboplastin solution, thromboplastin buffer, HEPES buffer, reagents (Rhodamine, Calcium Chloride, Tyloxapol, UV dye, Polybrene in HEPES, TDR), operated coating machine, performed the Web variability testing, blood testing, control solution testing, aware BBP & MSDS, assisted the R & D, inspected completed strips before pouching and sealing, and calibrated micropipettes.
• Manufacturing Duties: GMP, monitored daily temperature, performed optical comparison, made white cards, wrote Nonconformance Report, printed out labels with correct lot numbers, and part numbers.
Interviewed and trained new people.

Education:
BS, Biochemistry December 1998, UNIVERSITY OF CALIFORNIA AT DAVIS, CA.

Skills:
• PCR
• cDNA synthesis & cloning
• Gel Electrophoresis (argarose, formaldehyde, polyacrylamide)
• Bacterial Cell Culturing
• Spectrophotometry
• Agar Plate preparation
• Reagent Preparation
• Protein purification
• Antibody purification
• TLC
• HPLC
• Ion Exchange Chromatography
• SDS PAGE
• Mass Spectrophotography
• UV/Vis spectrophotography
• Affinity chromatography
• Western - dot blots
• Recrystallization

Additional Information:
EQUIPMENTS / SOFTWARES KNOWLEDGE

• Basic programming using Visual Basic
• Programming using FileMaker Pro
• Microsoft Word, Windows, Excel, PowerPoint
• Strong in Calculus
• Strong Chemistry background
• NMR – IR
• Thermocycler
• gel apparatus
• spectrophotometer
• lyophilizor
• incubator
• Eppendorf dispenser
• Eppendorf centrifuge 5810R
• Amersham Phast Gel system
• 96 well plate microplate reader with running program
• Omnispense
• Centrifuge
• gel imaging system
• pH and conductivity meters
• analytical balance

Candidate Contact Information:

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